Fly Facility

Fly-Facility is the microinjection and transgenesis platform localized at GReD, U1103 INSERM–UMR6296 CNRS, University of Clermont Auvergne. Fly-Facility was created in 2005 as Technological Platform of the European Network Myores. Growing interest in generation of transgenic Drosophila lines and high number of external requests lead to opening of Fly-Facility services, in spring 2008, to customers from outside the network. The longstanding expertise in transgenesis allowed to gain international visibility with referencing in « Ressources » web pages of the FlyBase, the major web tool and database of Drosophila research community. Finally from 2011, Fly-Facility join the National Infrastructure Network TEFOR, with objectives to develop new tools based on transgenesis and genome editing approaches.

• Microinjection and generation of transgenic Drosophila lines.
• Development and application of knockout and knock-in approaches using CRISPR/Cas9 technologies.
• Technological and research consulting in the field of Drosophila transgenesis and genome editing.

2 microinjection stations for cells and embryos

• Zeiss Axiovert inverted microscope with two joystic drived micromanipulators and an Eppendorf microinjector.
• Leica inverted microscope with two joystic drived micromanipulators and an Eppendorf microinjector.

Stereo microscope/macroscope Olympus SZX12 for fly pushing and embryo alignment. Possiblity to view and to capture transmitted light and GFP images.

3 thermo-regulated incubators for transgenesis.

• Microinjection and generation of transgenic Drosophila lines.
• Development and application of knockout and knock-in approaches using CRISPR/Cas9 technologies.
• Technological and research consulting in the field of Drosophila transgenesis and genome editing.

Training of PhD students, ERASMUS students and master students on transgenesis technologies and genome editing using CRISPR/Cas9.

Celphedia, Tefor