Cell Imaging Service (CICS)

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About us

The Centre Imagerie Cellulaire Santé (CICS) offers three complementary technical platforms to research lab and industrials:
  • electron microscopy (TEM and SEM) + sample preparation
  • photon microscopy (MP) + sample preparation
  • Flow cytometry (CMF)
The attached team consists of an engineer, an assistant engineer, a technician and a technical assistant to support the users.

Our services

  • Four full-time staff
  • Full support from sample preparation to image interpretation
  • Training in the use of imaging equipment and sample preparation
  • Scientific and technical watch
  • Our main themes = Animal / Plant Biology, Health, Pharmaceuticals / Cosmetics, Environment, Nutrition / Agrifood, Biomaterials, Nanotechnologies, Metallurgy, etc.

Facilities / Equipments

Located on 330m² on the ground floor of UFR Medicine, the CICS offers 3 technical rooms equipped with automated sample preparers and imaging equipments:
  • Hitachi H7650 Transmission Electron Microscope + Hamamatsu Camera
  • Jeol 6060LV Scanning Electron Microscope
  • BD LSR II Flow Cytometer
  • Olympus BX51 Light-field Microscope + Color View Camera
  • Olympus BX51 Fluorescence Microscope + CoolSnapES Camera

Some of our achievements

  • 1,2,3‐Triazolium‐Based Cationic Amphipathic Peptoid Oligomers Mimicking Antimicrobial Helical Peptides. Dr. Radhe Shyam, Nicolas Charbonnel, Aurélie Job, Christelle Blavignac, Christiane Forestier, Claude Taillefumier, Sophie Faure. ChemMedChem. 19 June 2018.
  • Development and cytotoxic response of two proliferative MDA-MB-231 and non-proliferative SUM1315 three-dimensional cell culture models of triple-negative basal-like breast cancer cell lines. Dubois Clémence, Dufour Robin, Daumar Pierre, Aubel Corinne, Szczepaniak Claire, Blavignac Christelle, Mounetou Emmanuelle, Penault-Llorca Frédérique and Mahchid Bamdad. Oncotarget 2017.
  • Absence of Fungal Spore Internalization by Bronchial Epithelium in Mouse Models Evidenced by a New Bioimaging Approach and Transmission Electronic Microscopy. Am J Pathol. 2015. Rammaert B, Jouvion G, de Chaumont F, Garcia-Hermoso D, Szczepaniak C, Renaudat C, Olivo-Marin JC, Chrétien F, Dromer F, Bretagne S.
  • Roles of capsule and lipopolysaccharide O antigen in interactions of human monocyte-derived dendritic cells and Klebsiella pneumoniae. Evrard B, Balestrino D, Dosgilbert A, Bouya-Gachancard JL, Charbonnel N, Forestier C, Tridon A. Infect Immun. 2010.

Training

  • DIU Electron Microscopy « normal and pathological ultrastructure »
  • Training in the use of imaging equipment and sample preparation