Gentyane Platform : High Throughput Genotyping and Sequencing in Auvergne

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About us

team
The Gentyane Genomic Services platform in Genotyping and High Throughput Sequencing, is attached to the UMR INRA-UCA 1095 Genetics Diversity and Ecophysiology of Cereals. Gentyane develops:
  1. a research and development activity on genotyping and sequencing technologies in connection with the research teams of UMR GDEC;
  2. a genotyping and sequencing service delivery activity for other INRA teams or other public and private bodies;
  3. a technological watch allowing to put in place new tools answering better, more quickly and at a lower cost to the expectations of the users.
The platform is certified ISO 9001: 2015 and NF-X 50-900 2016, labeled Collective Scientific Infrastructure in September 2018.

Our services

Services that may be offered:

Delivery in high-speed production:

  • Genotyping: Microsatellites (SSR), Single Nucleotide Polymorphism (SNP), Structural Variations (CNV)
  • Sequencing: Long Reads (PacificBiosciences Sequel 1M and 8M)
  • Optical Mapping: Optical Maps (Saphyr BionanoGenomics)

Proposed Genomic Applications:

  • Sequencing and assembly of complex genomes
  • Structural Variant Analysis (SNP Single Nucleotide Polymorphism, SSR Single Sequence Repeat, CNV Copy Number Variation) for Phylogenetic Diversity Characterization
  • Expression analysis.

Genomic applications in development:

  • Sequencing complex genomes: combination of techniques to obtain an innovative service: Pacific Biosciences Sequel 8M + Bionano Genomics Saphyr (and / or TEN X Genomics). Combined with conventional bioinformatic tools for genome assembly (in connection with the SIBI platform), this service will ultimately provide a turnkey service for analyzing the structure of any complex genome.

Technologies / Equipments

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Equipments
Trademark Model
Affymetrix Genetitan 1 AFFYMETRIX Axiom
Affymetrix Genetitan 2 AFFYMETRIX Axiom
BIOMARK FLUIDIGM BIOMARK
BIOMEK FX dual 96/384 BECKMAN FXp
BIOMEK FXp dual span8 Affimetrix BECKMAN FXp
OMEK FXp dual span8 (upgrade du FX) BECKMAN FXp
Blue pipin Sage Science Blue Pippin
Broyeur à plaques SPECKS GenoGrinder 2010
Covaris Kbioscience M220
Dropsense 16 Proteigene Dropsense16
Electrophorese 96 capillaires APPLIED BIO ABI 3730XL
Fragment Analyser 12 Advanced Analytical Fragment Analyser 12
Fragment Analyser 96 Advanced Analytical Fragment Analyseur 96
HAMILTON STAR HAMILTON STAR
HAMILTON STARLET HAMILTON STARLET
JUNO Fluidigm JUNO System
LC480 ROCHE LightCycler 480 II
Lecteur de plaques TECAN Infinite 1000
Megaruptor Diagenode Megatruptor
Pippin pulse Sage Science Pippin pulse
Robot Extraction ADN Oktopure LGC Genomics Oktopure
SEQUEL 1M Pacific biosciences SEQUEL 1M
SEQUEL 8M Pacific biosciences SEQUEL 8M
5 thermocycleurs 384 Eppendorf Mastercycler pro 384
111 thermocycleurs 384 APPLIED BIO VERITI
3 thermocycleurs 96 APPLIED BIO VERITI
Thermosoudeuse automatique AGILENT PlateLoc
Viaflo 96 INTEGRA VIAFLO96
FemtoPulse Advanced Analytical FemtoPulse
Saphyr Bionanogenomics Saphyr
Robot pipeteur I7 BECKMAN I7

Some of our achievements

  • Realization of 622 projects in 10 years
  • Collaboration in many PIA projects: BreedWheat, Amaizing, Sunrise ...
  • Collaboration in many European projects FEAMP: Genesea, NeoBio, S'Sturgeon ...

Partnerships

Gentyane is service provider for Pacific Biosciences Sequencing Technologies, Bionano Genomics Optical Card and Axiom Affymetrix Chip Genotyping.