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Platform of Exploration of Metabolism (PFEM)
About us
Platform of "Exploration of Metabolism" (PFEM) is a technological and scientific platform that supports metabolomic and proteomic projects. This INRAE-UCA joint infrastructure obtained the French IBiSA labelisation in 2013 and NF X50-900 certification in 2015. PFEM is member of the French National Facility in metabolomics and proteomics (PIA) MetaboHUB (MTH ; www.metabohub.fr) wich aims to develop tools, methodologies and support major projects involving metabolomic and fluxomic approaches.
Our services
Analysis of biological fluids (urine, plasma, microorganism extracts ...) by LC-MS / NMR. Standardized and automated sample preparation.
- Determination of metabolic profils by LC-MS/NMR
- Data set processing and statistic studies.
- Identification of biomarkers by LC-MSMS and 2D NMR
- LC-NMR analysis
Equipments
- Robot for sample preparation for LC-MS and NMR analysis
- ESI-Tof mass spectrometer, (micrOTOF Bruker) coupled to a Fast-LC (1200 series, Agilent)
- AVANCE III Ultrashield NMR spectrometer equipped with a cryoprobe (Bruker)
Contacts
Scientific leader PFEM
Estelle Pujos-Guillot
Tel.: +33(0)4 73 62 41 41
Tel.: +33(0)4 73 62 41 41
Technical expert PFEM
Stéphanie Durand
Tel.: +33(0)4 73 62 41 55
Scientific leader Proteomic
Michel Hébraud
Tel.: +33(0)4 73 62 46 70
Technical expert Proteomic
Christophe Chambon
Tel.: +33(0)4 73 62 44 64